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Updated: Sep 12, 2026

Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy
Published on: September 3, 2017
Two-photon imaging of formaldehyde in live cells and animals utilizing a lysosome-targetable and acidic
Xilei Xie1, Fuyan Tang, Xiaoyan Shangguan
1College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China. tangb@sdnu.edu.cn wangxu@sdnu.edu.cn.
Abstract:
Lyso-TPFP presents lysosomal targetability and an acidic pH-activatable response toward formaldehyde. Thus, it exclusively visualizes lysosomal formaldehyde and is immune against it in neutral cytosol and other organelles. In addition, two-photon fluorescence imaging endows Lyso-TPFP with the capability of in situ tracking formaldehyde in live cells and animals.
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