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Related Experiment Video

Updated: Mar 1, 2026

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Published on: May 13, 2020

Acknowledging Errors: Advanced Molecular Replacement with Phaser.

Airlie J McCoy¹

¹Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust/MRC Building, Hills Road, Cambridge, CB2 0XY, UK. ajm201@cam.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|June 3, 2017

Summary

This summary is machine-generated.

Molecular replacement (MR) is a key method for solving the crystallographic phase problem. Advanced techniques now enable MR for challenging structures, making it widely used for protein structure determination.

Keywords:

LLGI Maximum likelihood Molecular replacement

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Area of Science:

Structural Biology
Crystallography
Biophysics

Background:

Molecular replacement (MR) is a fundamental technique for solving the crystallographic phase problem.
Historically, MR was limited to cases with high homology between model and target structures.
Recent advancements have expanded the applicability of MR to more complex scenarios.

Purpose of the Study:

To review the evolution and current state of molecular replacement phasing.
To highlight the impact of advanced statistical methods on MR.
To discuss strategies for addressing challenging MR cases.

Main Methods:

Application of maximum likelihood statistics in MR.
Development of state-of-the-art computational approaches.
Analysis of challenging cases at the limits of MR solvability.

Main Results:

MR has become the dominant method for structure determination, accounting for most Protein Data Bank entries.
Maximum likelihood methods allow for handling model errors and data pathologies.
Previously intractable cases are now routinely solvable using modern MR techniques.

Conclusions:

Molecular replacement is a powerful and versatile tool in structural biology.
Ongoing research focuses on pushing the boundaries of MR for difficult structural targets.
Advanced MR approaches are crucial for expanding our understanding of biological macromolecules.