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Draft genome sequence of a Kluyvera intermedia isolate from a patient with a pancreatic abscess
Roland Thele1, Heidi Gumpert1, Louise B Christensen1
1Department of Clinical Microbiology, Hvidovre Hospital, University of Copenhagen Hvidovre, Denmark.
Abstract:
The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain was reported as sensitive to Danish-registered antibiotics although it had a fosA-like gene in the genome. There were nine contigs that aligned to a plasmid, and these contigs contained several heavy metal resistance gene homologues. Furthermore, a prophage was discovered in the genome. WGS represents an efficient tool for monitoring Kluyvera spp. and its role as a reservoir of multidrug resistance. Therefore, this susceptible K. intermedia genome has many characteristics that allow comparison of resistant K. intermedia that might be discovered in the future.
Insights
This study sequenced the genome of a Kluyvera intermedia strain, revealing antibiotic sensitivity despite carrying resistance genes. This finding aids future comparisons with multidrug-resistant Kluyvera strains.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- The genus Kluyvera includes opportunistic pathogens causing diverse infections.
- Kluyvera species can act as reservoirs for antimicrobial resistance genes.
- Understanding Kluyvera genomes is crucial for infection control.
Purpose of the Study:
- To characterize a Kluyvera intermedia strain isolated from a pancreatic cyst.
- To investigate the genomic features, including antibiotic and heavy metal resistance genes, and prophage presence.
- To establish a reference genome for comparison with future multidrug-resistant strains.
Main Methods:
- Whole-genome sequencing (WGS) of the Kluyvera intermedia isolate (FOSA7093).
- Bioinformatic analysis to identify genes, plasmids, and prophages.
- Phenotypic antibiotic susceptibility testing against Danish-registered antibiotics.
Main Results:
- The K. intermedia strain FOSA7093 was sensitive to tested antibiotics.
- The genome contained a fosA-like gene, suggesting potential for resistance.
- Nine plasmid contigs with heavy metal resistance gene homologues and a prophage were identified.
Conclusions:
- Whole-genome sequencing is an effective method for monitoring Kluyvera species.
- This susceptible K. intermedia genome provides a valuable baseline for studying multidrug resistance evolution.
- Further research is needed to understand the clinical significance of resistance genes in Kluyvera.
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