Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals01:17

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

3.6K
Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...
3.6K
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

678
Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
678
Positron Emission Tomography01:29

Positron Emission Tomography

7.8K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Food and Drug Administration boxed warning on menopausal hormone therapy: history, impact, and a regulatory inflection point in women's health.

Menopause (New York, N.Y.)·2026
Same author

Psychological Aspects and Implications of Food Addiction and Glucose Control in Type 2 Diabetes: A Pilot Mixed-Methods Study.

Healthcare (Basel, Switzerland)·2026
Same author

A Novel Approach to In Vivo EPR Spectroscopy for Repeatable Assessments of Oxygenation Levels in Tumors at Any Depth: Preliminary Feasibility Studies Utilizing a Multisite Oxygen Sensor Inside HDR Brachytherapy Needles.

Advances in experimental medicine and biology·2026
Same author

What Is the Information Content of EPR Oximetry?

Advances in experimental medicine and biology·2026
Same author

Letting Biology Validate the EPR Imaging of Tumor pO<sub>2</sub>.

Advances in experimental medicine and biology·2026
Same author

The Future is Now - Emerging Trends in the Older Adult.

Journal of human nutrition and food science·2025
Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
See all related articles

Related Experiment Video

Updated: Mar 1, 2026

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.9K

Rapid-scan EPR imaging.

Sandra S Eaton1, Yilin Shi1, Lukas Woodcock1

  • 1Department of Chemistry and Biochemistry and Center for EPR Imaging In Vivo Physiology, University of Denver, Denver, CO 80210, United States.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|June 6, 2017
PubMed
Summary
This summary is machine-generated.

Rapid-scan Electron Paramagnetic Resonance (EPR) spectroscopy offers enhanced signal-to-noise ratios by scanning magnetic fields or frequencies quickly. This advanced technique improves upon conventional continuous wave EPR, especially for low-frequency EPR imaging applications.

Keywords:
DeconvolutionDirect detectionImagingIn vivoNitroxides

More Related Videos

Highly-Multiplexed Tissue Imaging with Raman Dyes
07:18

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

3.4K
In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

8.8K

Related Experiment Videos

Last Updated: Mar 1, 2026

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.9K
Highly-Multiplexed Tissue Imaging with Raman Dyes
07:18

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

3.4K
In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

8.8K

Area of Science:

  • Electron Paramagnetic Resonance (EPR) Spectroscopy
  • Advanced Spectroscopic Techniques

Background:

  • Conventional continuous wave EPR has limitations in speed and signal-to-noise ratio.
  • Rapid-scan EPR (RS-EPR) addresses these limitations by employing faster scan rates.
  • Understanding electron spin relaxation rates is crucial for optimizing RS-EPR.

Purpose of the Study:

  • To provide an overview of the principles and hardware of rapid-scan EPR.
  • To highlight the advantages of RS-EPR over conventional continuous wave EPR.
  • To demonstrate the application of RS-EPR in imaging various radical species.

Main Methods:

  • Implementing rapid scanning of magnetic field or frequency.
  • Direct signal detection using a mixer at the source frequency.
  • Utilizing mathematical deconvolution to process spectral oscillations and recover absorption spectra.
  • Applying RS-EPR technology within the linear response region.

Main Results:

  • RS-EPR achieves substantial signal-to-noise improvement compared to conventional EPR.
  • Oscillations in rapid scans can be mathematically deconvoluted to obtain accurate spectra.
  • The technique allows for higher microwave power usage without saturation.
  • Successful application in imaging nitroxide radicals, diradicals, and spin-trapped radicals at 250MHz.

Conclusions:

  • Rapid-scan EPR is a powerful technique offering significant advantages in signal-to-noise and spectral quality.
  • Deconvolution methods enable accurate spectral recovery even with scan-induced artifacts.
  • RS-EPR shows particular promise for low-frequency EPR imaging applications, enhancing discoverability.