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Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA
B E Tarn1, K-J Sung1, H D Sikes1
1Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.
Molecular Systems Design & Engineering
|June 6, 2017
Abstract:
Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant that binds hemi-methylated CpG dinucleotides.
