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Estimation of Guggulsterone E and Z in the Guggul-based Commercial Formulations Using High-performance Thin-layer
Pramod Kumar Sairkar1, Anjana Sharma2, N P Shukla3
1Centre of Excellence in Biotechnology, M. P. Council of Science and Technology, Bhopal, Madhya Pradesh, India.
Journal of Pharmacy & Bioallied Sciences
|June 7, 2017
Summary
This study quantifies Guggulsterone (GS) isomers in herbal formulations using HPTLC. Results show varying E-GS and Z-GS concentrations, aiding quality control for traditional Ayurvedic medicines.
Area of Science:
- Phytochemistry
- Analytical Chemistry
- Pharmacognosy
Background:
- Guggulsterone (GS) is a bioactive plant steroid from Commiphora wightii (gum Guggul).
- Ayurveda utilizes C. wightii for various medicinal purposes.
- Complex phytochemistry and variable availability challenge GS analysis.
Purpose of the Study:
- To estimate Guggulsterone (GS) and its E/Z isomers in six herbal formulations.
- To employ high-performance thin-layer chromatography (HPTLC) for quantitative analysis.
Main Methods:
- HPTLC analysis of E-GS and Z-GS standards and samples.
- Mobile phase: toluene: ethyl acetate: formic acid: methanol (6:2:1:0.5).
- Detection via UV scanning at 254 nm, with absorbance spectra from 200-400 nm.
Main Results:
- Rf values and scan spectra confirmed the presence of E-GS and/or Z-GS in formulations.
- Quantified E-GS ranged from 0.230 ± 0.0040% to 0.926 ± 0.0168%.
- Quantified Z-GS ranged from 0.537 ± 0.0026% to 0.723 ± 0.0177%.
Conclusions:
- HPTLC effectively quantifies E-GS and Z-GS in herbal products.
- The study provides crucial data for quality control of Guggul-based medicines.
- Findings support the standardization of Ayurvedic formulations containing Guggulsterone.
