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T-Cell-Mediated Cross-Protective Immunity.

Nina Khanna1,2, Claudia Stuehler3

  • 1Division of Infectious Diseases and Hospital Epidemiology, Departments of Biomedicine and Clinical Research, University Hospital of Basel, Basel, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|June 7, 2017
PubMed
Summary
This summary is machine-generated.

Developing T cells that target multiple fungal pathogens is crucial for effective fungal infection treatments. This study presents a new ELISpot method to assess T cell cross-reactivity against diverse molds and yeasts, aiding vaccine and cell therapy development.

Keywords:
Cross-reactive T cellsDendritic cellsELISpotHeat-inactivated fungiT cell clonesT cell lines

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Area of Science:

  • Immunology
  • Microbiology
  • Infectious Diseases

Background:

  • Invasive fungal infections pose a significant threat, caused by diverse yeast and mold species.
  • Current treatments face challenges due to the variety of fungal pathogens.
  • T cells recognizing conserved fungal epitopes are promising for immunotherapy.

Purpose of the Study:

  • To develop and validate an ELISpot-based assay for assessing T cell cross-reactivity.
  • To evaluate the potential of T cells in targeting multiple fungal species.
  • To support the development of novel vaccination strategies and adoptive T cell therapies against fungal infections.

Main Methods:

  • Enzyme-Linked ImmunoSpot (ELISpot) assay development.
  • Testing T cell lines and clones for reactivity to various fungal antigens.
  • Utilizing conserved fungal epitopes for broad T cell recognition.

Main Results:

  • Demonstrated the feasibility of using ELISpot to measure T cell responses to fungal pathogens.
  • Showcased the cross-reactivity of specific T cells against different mold and yeast species.
  • Identified T cell populations with potential for broad antifungal activity.

Conclusions:

  • The described ELISpot method is effective for evaluating T cell cross-reactivity against fungal pathogens.
  • This assay can facilitate the identification of T cells suitable for broad-spectrum antifungal therapies.
  • The findings support the advancement of T cell-based strategies for managing invasive fungal infections.