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Updated: Mar 1, 2026

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
Published on: June 27, 2020
Cytokine Expression by T Follicular Helper Cells
Christoph Jandl1,2, Claudia Loetsch1,2, Cecile King3,4
1Department of Immunology, Garvan Institute of Medical Research, 384 Victoria St., Darlinghurst, NSW, 2010, Australia.
This study presents a flow cytometry method to detect intracellular cytokines in T follicular helper (Tfh) cells. This technique aids in understanding Tfh cell responses in immunity and autoimmunity.
Area of Science:
- Immunology
- Cell Biology
Background:
- T follicular helper (Tfh) cells are crucial for adaptive immunity, residing in germinal centers (GC) within lymphoid organs.
- Tfh cells support high-affinity antibody production via cytokine secretion (e.g., IL-21, IL-4) and cell interactions.
Purpose of the Study:
- To describe a flow cytometry-based method for detecting intracellular cytokines within Tfh cells.
- To enable the study of Tfh cell responses in the context of immunity and autoimmunity.
Main Methods:
- Utilized flow cytometry to analyze intracellular cytokine production.
- Focused on Tfh cell populations within secondary lymphoid organs (spleen, lymph nodes, Peyer's patches).
Main Results:
- Successfully detected intracellular cytokines (IL-21, IL-4) within the Tfh cell population.
- Demonstrated the method's applicability to various secondary lymphoid organs.
Conclusions:
- The described method facilitates the investigation of Tfh cell function and responses.
- This technique is valuable for studying immune and autoimmune conditions involving Tfh cells.
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