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Follicular Dendritic Cell Isolation and Loading of Immune Complexes
Balthasar A Heesters1, Cees E Van der Poel1, Michael C Carroll2
1Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, 02115, USA.
Abstract:
Follicular dendritic cells (FDCs) are stromal cells that are centrally located within B cell follicles of lymph nodes and other lymphoid organs such as the spleen. Due to their relative low abundance and difficulty to isolate, FDCs are still largely an enigma. Here we describe how to isolate FDCs for ex vivo cell culture, sorting by flow cytometry and how to load them in vivo or in vitro with immune complexes.
