Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

16.7K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
16.7K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

9.5K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiple broadly neutralizing antibody lineages can co-exist and mature in the same germinal centers.

Cell reports·2026
Same author

Delayed type I interferon response and the subsequent out-of-sequence cytokine signal inhibit T cell induction in non-surviving Ebola virus-infected patients.

Frontiers in immunology·2026
Same author

Guideline-based strategies to identify severe cytokine release syndrome in COVID-19 and cancer immunotherapy using large-scale electronic health records.

Frontiers in digital health·2026
Same author

Lymphatic constraint of germinal centers optimizes protective antibody responses.

bioRxiv : the preprint server for biology·2025
Same author

Transient silencing of hypermutation preserves B cell affinity during clonal bursting.

Nature·2025
Same author

Hybrid metapopulation agent-based epidemiological models for efficient insight on the individual scale: A contribution to green computing.

Infectious Disease Modelling·2025
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Mar 1, 2026

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.5K

How to Simulate a Germinal Center.

Philippe A Robert1,2, Ananya Rastogi3, Sebastian C Binder3

  • 1Systems Immunology Department and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38126, Braunschweig, Germany. philippe.robert@ens-lyon.org.

Methods in Molecular Biology (Clifton, N.J.)
|June 8, 2017
PubMed
Summary
This summary is machine-generated.

This study details the hyphasma agent-based model for simulating germinal center dynamics and B cell affinity maturation. The model enables reliable predictions for improving vaccines and understanding immune memory against pathogens.

Keywords:
Affinity maturationAgent-based modelingGerminal centerSomatic hypermutation

More Related Videos

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.6K
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
09:35

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

7.3K

Related Experiment Videos

Last Updated: Mar 1, 2026

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.5K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.6K
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
09:35

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

7.3K

Area of Science:

  • Immunology
  • Computational Biology
  • Systems Biology

Background:

  • Germinal centers (GCs) are crucial microenvironments for B cell affinity maturation, essential for adaptive immunity and immunological memory.
  • This process involves B cell receptor mutation and selection based on antigen affinity, leading to high-affinity antibodies for pathogen clearance.
  • Therapeutic targeting of GCs offers potential for vaccine enhancement and control of chronic infections like HIV, but GC dynamics are complex.

Purpose of the Study:

  • To present a detailed methodology for constructing an agent-based model (hyphasma) that simulates germinal center dynamics.
  • To incorporate key quantitative traits of affinity maturation within the model.
  • To demonstrate the model's capability for making reliable predictions regarding GC processes.

Main Methods:

  • Development of an agent-based model (hyphasma) to simulate the microenvironment and cellular interactions within germinal centers.
  • Inclusion of core quantitative aspects of B cell receptor affinity maturation, including mutation and selection dynamics.
  • Validation of the model's predictive power through comparison with previous study outcomes.

Main Results:

  • The hyphasma model successfully replicates the essential dynamics of germinal center reactions.
  • The model quantitatively captures the process of B cell affinity maturation, from low to high affinity.
  • Previous studies utilizing this model have demonstrated its capacity for making reliable predictions.

Conclusions:

  • The agent-based model hyphasma provides a robust framework for studying germinal center dynamics and affinity maturation.
  • This computational tool can aid in understanding immune memory and developing novel therapeutic strategies.
  • Hyphasma facilitates reliable predictions, advancing research in immunology and vaccine development.