Related Experiment Video
Updated: Mar 1, 2026
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
Published on: August 31, 2014
Single-Molecule Threshold of HIV Fate Decision
1Department of Integrative Biology and Physiology, Department of Chemistry and Biochemistry, and Institute of Quantiative and Computational Biology, University of California - Los Angeles, Los Angeles, California.
Abstract:
During early infection, the HIV virus makes a key decision between two states: lytic and lysogenic fate. Deterministic bistability requires combination of positive feedback and ultrasensitivity. Although HIV circuity includes positive feedback activation of the Tat transactivator, it lacks ultrasensitivity. How does the HIV circuit allow for multiple fates without ultrasensitivity? A new article suggests that HIV bistability is a result of a transient threshold that allows the kinetic trapping of the inactive state. Interestingly, the model shows that the transient threshold is a result of a single molecule threshold that occurs when the promoter toggles between inactive and active states.
More Related Videos
07:15Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform
Published on: May 18, 2021
13:07Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Published on: January 30, 2019
