Differential expression between "DSP-only" and DSP-PP523 transcripts in rat molar teeth
Ya-Qin Zhu1, Ryan M Song2, Helena H Ritchie2
1Department of General Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Archives of Oral Biology
|June 9, 2017
Summary
The DSP-only transcript and DSP-PP523 transcript show different expression patterns during tooth development. DSP-only transcript is found in the dental pulp and below mature odontoblasts, suggesting distinct roles in dentinogenesis.
Area of Science:
- Biochemistry
- Developmental Biology
- Genetics
Background:
- The dentin sialophosphoprotein (DSPP) gene yields multiple transcripts, including DSP-only and DSP-PP523.
- Understanding the differential expression of these transcripts is crucial for elucidating their specific roles in tooth development.
Purpose of the Study:
- To compare the expression patterns of the DSP-only and DSP-PP523 transcripts during molar tooth development.
- To investigate the potential distinct functions of these transcripts in dentinogenesis.
Main Methods:
- Utilized in situ hybridization with unique riboprobes to detect DSP-only and DSP-PP523 transcript expression in developing rat molars.
- Analyzed paraffin-embedded molar sections from various embryonic and postnatal stages.
- Employed immunohistochemistry to identify Stro-1 protein expression, a stem cell marker.
Main Results:
- DSP-PP523 transcript expression was observed in mature odontoblasts involved in dentin formation.
- DSP-only transcript was detected in the dental pulp and subjacent to the mature odontoblast layer, not within the odontoblasts themselves.
- Stro-1 protein, a stem cell marker, was also found in the dental pulp and areas subjacent to odontoblasts.
Conclusions:
- The differential expression patterns of DSP-only and DSP-PP523 transcripts suggest they have distinct functions during dentinogenesis.
- DSP-PP523 transcript is associated with active dentin formation by mature odontoblasts.
- DSP-only transcript may play a different role, potentially related to stem cell populations in the dental pulp.
