Investigation on intermolecular interaction between berberine and β-cyclodextrin by 2D UV-Vis asynchronous spectra
Anqi He1, Xiaoyan Kang2, Yizhuang Xu3
1Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China; Ninghai Doubly Advanced Material Co, Ltd., Ninghai 315602, PR China; Department of Chemistry, School of Science, Kwansei Gakuin University, Sanda, Hyogo 669-1337, Japan.
Summary
Berberine chloride and beta-cyclodextrin (β-CyD) interact, confirmed by 2D UV-Vis spectroscopy. This interaction causes spectral shifts, explaining enhanced berberine solubility.
Area of Science:
- Analytical Chemistry
- Physical Chemistry
- Spectroscopy
Background:
- Berberine chloride is a natural compound with therapeutic potential.
- Beta-cyclodextrin (β-CyD) is a cyclic oligosaccharide used to enhance drug solubility.
- Understanding the interaction mechanism between berberine and β-CyD is crucial for drug formulation.
Purpose of the Study:
- To investigate the intermolecular interaction between berberine chloride and β-cyclodextrin (β-CyD).
- To elucidate the spectral changes associated with this interaction.
- To provide insights into the enhanced solubility of berberine by β-CyD.
Main Methods:
- Two-dimensional (2D) asynchronous ultraviolet-visible (UV-Vis) spectroscopy was employed.
- Analysis of cross peaks in the 2D asynchronous spectrum.
- Monitoring spectral shifts and bandwidth changes of berberine bands.
Main Results:
- The 2D asynchronous UV-Vis spectrum showed cross peaks, confirming intermolecular interaction between berberine chloride and β-CyD.
- A red-shift and decreased bandwidth of the 420nm band of berberine were observed.
- The spectral changes indicate a shift towards a more hydrophobic environment for berberine.
Conclusions:
- Specific intermolecular interactions occur between berberine chloride and β-CyD.
- The observed spectral changes, particularly the red-shift of the n-π* transition band, explain the enhanced solubility of berberine in the presence of β-CyD.
- This study provides valuable spectral evidence for the complexation mechanism.
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