Characterization of Hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: Optimization...

Area of Science:

• Phytochemistry
• Food Science
• Natural Product Chemistry

Background:

• Hypericum perforatum (HP) is a medicinal plant with potential bioactive compounds.
• Polysaccharides are known for their diverse biological activities.
• Optimization of extraction parameters is crucial for maximizing yield and efficacy.

Purpose of the Study:

• To optimize the extraction parameters for crude polysaccharides (CPSs) from Hypericum perforatum (HP).
• To characterize the extracted polysaccharides using Fourier transform-infrared spectroscopy (FT-IR).
• To evaluate the antioxidant and antibacterial activities of the HP-CPSs.

Main Methods:

• Box-Behnken design-response surface methodology (BBD-RSM) was employed for parameter optimization.
• Key extraction parameters optimized: extraction time (ETi), extraction temperature (ETe), and water/solid ratio (W/S).
• Antioxidant activity assessed via DPPH and OH radical scavenging assays.
• Antibacterial activity evaluated against various bacterial strains (E. coli, S. dysenteriae, S. typhi, B. cereus, S. aureus).

Main Results:

• Optimal extraction conditions determined as: ETi 117.5 min, ETe 74.28°C, and W/S 20.3:1.
• The experimental yield of HP-CPSs under optimal conditions was 6.69%.
• FT-IR analysis confirmed the polysaccharide structure.
• HP-CPSs exhibited significant in vitro antioxidant and antibacterial activities against tested pathogens.

Conclusions:

• The optimized extraction method effectively yields bioactive polysaccharides from HP.
• HP-CPSs possess notable antioxidant and antibacterial properties.
• These findings suggest HP-CPSs are promising candidates for antioxidant ingredients in the food industry.