Assessment of nanoparticles' safety: corrected absorbance-based toxicity test

Area of Science:

• Nanotechnology
• Toxicology
• Cell Biology

Background:

• Absorbance-based toxicity tests are widely used but can be confounded by nanoparticle deposition.
• Accurate assessment of nanoparticle toxicity is crucial for safe application in various fields.

Purpose of the Study:

• To develop and validate a method for correcting absorbance-based toxicity tests.
• To eliminate the influence of deposited nanoparticle dose on toxicity assay results.
• To ensure reliable cell viability measurements in nanoparticle toxicity studies.

Main Methods:

• A novel correction method was developed for absorbance-based toxicity assays.
• The widely used MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was modeled.
• Corrected cell viability data was compared against fluorescence-based PrestoBlue® assay results.

Main Results:

• The developed method effectively corrected for the deposited dose of nanoparticles in absorbance-based tests.
• Corrected cell viability data from the MTS assay showed strong agreement with PrestoBlue® assay results.
• This indicates the successful removal of interference from nanoparticle deposition.

Conclusions:

• The novel correction method enhances the accuracy of absorbance-based nanoparticle toxicity testing.
• This approach provides a reliable alternative for assessing cell viability in the presence of nanoparticles.
• The findings support the use of corrected absorbance assays for robust nanotoxicology evaluations.