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Published on: June 28, 2024
A single quantum dot-based nanosensor for the signal-on detection of DNA methyltransferase
Fei Ma1, Wen-Jing Liu, Bo Tang
1College of Chemistry, Chemical Engineering, Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular, Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn tangb@sdnu.edu.cn.
Abstract:
We develop a single quantum dot (QD)-based nanosensor for the signal-on detection of DNA methyltransferase (MTase). By integration of single-molecule counting with the QD-based fluorescence resonance energy transfer (FRET), the proposed nanosensor can sensitively detect DNA MTase with a detection limit of as low as 0.002 U mL-1, and it can be further applied for inhibitor screening and accurate detection of DNA MTase in complex biological samples.

