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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
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An oxime-based glycocluster microarray.

Antoine Hoang1, Eugénie Laigre2, David Goyard2

  • 1LETI-CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.

Organic & Biomolecular Chemistry
|June 13, 2017
PubMed
Summary
This summary is machine-generated.

We developed two new oxime-based methods for creating carbohydrate microarrays. These methods improve the detection of proteins and cells by presenting sugars in defined clusters, leading to stronger and more selective interactions.

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Area of Science:

  • Carbohydrate chemistry
  • Surface chemistry
  • Biotechnology

Background:

  • Carbohydrate microarrays are essential for studying carbohydrate-binding molecules.
  • Current methods for microarray fabrication can be complex and time-consuming.
  • Controlled presentation of carbohydrates is crucial for enhancing molecular recognition.

Purpose of the Study:

  • To develop novel, efficient oxime-based methods for carbohydrate microarray fabrication.
  • To create surfaces with well-defined carbohydrate structures and valency.
  • To improve the recognition potency of carbohydrate microarrays for lectins.

Main Methods:

  • Developed a 'direct' method involving coating aminooxylated glycoclusters onto aldehyde-activated SiO2 surfaces.
  • Developed an 'indirect' method using sequential oxime ligation for chemoselective sugar clustering.
  • Utilized binding assays with labeled lectins and Isothermal Titration Calorimetry (ITC) for interaction analysis.

Main Results:

  • Both methods successfully generated carbohydrate microarrays with defined structures and valency.
  • Clustered sugar presentation significantly enhanced the potency and selectivity of lectin interactions.
  • ITC confirmed the improved binding interactions observed in the microarray assays.

Conclusions:

  • Oxime-based chemistry provides robust and versatile strategies for carbohydrate microarray construction.
  • Clustered carbohydrate presentation on microarrays enhances molecular recognition events.
  • These methods offer improved tools for detecting carbohydrate-binding proteins, pathogens, and cells.