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Updated: Feb 28, 2026

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Published on: July 10, 2014
Effect of etching on bonding of a self-etch adhesive to dentine affected by amelogenesis imperfecta
Don Jeevanie Epasinghe1, Cynthia Kar Yung Yiu2
1Department of Applied Oral Sciences, Faculty of Dentisty, The University of Hong Kong, Prince Philip Dental Hospital, Hong Kong, China.
Aim:
Dentine affected by amelogenesis imperfecta (AI) is histologically altered due to loss of hypoplastic enamel and becomes hypermineralized. In the present study, we examined the effect of additional acid etching on microtensile bond strength of a self-etch adhesive to AI-affected dentine.
Methods:
Flat coronal dentine obtained from extracted AI-affected and non-carious permanent molars were allocated to two groups: (a) Clearfil SE Bond (control); and (b) Clearfil SE Bond and additional etching with 34% phosphoric acid for 15 seconds. The bonded teeth were sectioned into .8-mm2 beams for microtensile bond strength testing, and stressed to failure under tension. The bond strength data were analyzed using two-way analysis of variance (dentine type and etching step) and Student-Newman-Keuls multiple comparison test (P<.05). Representative fractured beams from each group were examined under scanning electron microscopy.
Results:
Both factors, dentine substrate (P<.001) and etching step (P<.05), and their interactions (P<.001), were statistically significant. Additional etching had an adverse effect on the bond strength of Clearfil SE Bond to normal dentine (P<.005), and no significant improvement was found for AI-affected dentine (P=.479).
Conclusion:
Additional acid etching does not improve the bond strength of a self-etch adhesive to AI-affected dentine.
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