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Flow Cytometric Characterization of Murine B Cell Development
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A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry
Katharina Pracht1, Julia Meinzinger1, Patrick Daum1
1Division of Molecular Immunology, Internal Medicine III, Nikolaus-Fiebiger-Center of Molecular Medicine, University Hospital Erlangen, Erlangen, Germany.
European Journal of Immunology
|June 14, 2017
Abstract:
We provide a robust four-color fluorescence-based flow cytometry protocol that distinguishes viable dividing plasmablasts from nondividing plasma cells and, based on CD19 surface abundance, identifies two mature plasma cell populations in the spleen and the bone marrow of mice.
