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Updated: Feb 28, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

A selection guide for the new generation 6-dye DNA profiling systems.

Sze-Wah Lin¹, Christina Li¹, Stephen C Y Ip¹

¹Forensic Science Division, Government Laboratory, Homantin Government Offices, Hong Kong, China.

Forensic Science International. Genetics

|June 14, 2017

Summary

The FBI expanded CODIS core loci to 20, enhancing international compatibility. Three new STR amplification kits were evaluated for performance in forensic DNA analysis.

Keywords:

Forensic DNA typing Fusion 6C GlobalFiler Identifiler plus Investigator 24plex

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Last Updated: Feb 28, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

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Published on: March 9, 2015

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

Forensic Science
Molecular Biology
Genetics

Background:

The Federal Bureau of Investigation (FBI) mandated an expansion of the CODIS core loci from 13 to 20.
This expansion aims to increase international compatibility and improve the efficiency of national DNA databases for law enforcement.

Purpose of the Study:

To evaluate and compare the performance of three new 6-dye STR amplification kits for forensic DNA profiling.
To determine the suitability of these kits for laboratories adopting the expanded CODIS core loci.

Main Methods:

Performance assessment of Investigator 24plex QS Kit (QIAGEN), GlobalFiler™ PCR Amplification Kit (Applied Biosystems™), and PowerPlex® Fusion 6C System (Promega).
Evaluation included sensitivity, inhibitor tolerance, heterozygous peak height, intra-color signal balance, minor component profiling in mixtures, and mixture ratio preservation.

Main Results:

GlobalFiler™ demonstrated the highest sensitivity.
Investigator 24plex QS Kit showed superior tolerance to PCR inhibitors (Humic acid, Tannic acid).
GlobalFiler™ and Fusion 6C provided better heterozygous peak height and intra-color signal balance. GlobalFiler™ and Investigator 24plex were better at profiling minor mixture components, while Fusion 6C maintained mixture ratios more consistently.

Conclusions:

The three evaluated STR amplification systems possess distinct performance characteristics.
Laboratories should select kits based on specific operational needs and requirements for forensic DNA analysis.