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Updated: Feb 28, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Fourier ptychographic microscopy using wavelength multiplexing.

You Zhou¹, Jiamin Wu¹, Zichao Bian²

¹Tsinghua University, Department of Automation, Beijing, China.

Journal of Biomedical Optics

|June 15, 2017

Summary

This summary is machine-generated.

We developed a wavelength multiplexing strategy to significantly accelerate Fourier ptychographic microscopy (FPM) acquisition times. This innovation enables high-speed gigapixel microscopy for dynamic imaging applications.

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Area of Science:

Microscopy
Optical Imaging
Computational Imaging

Background:

Fourier ptychographic microscopy (FPM) offers wide-field, high-resolution imaging by combining low-resolution images in the Fourier domain.
The slow image acquisition process of traditional FPM limits its use in imaging dynamic biological processes or rapidly changing samples.

Purpose of the Study:

To develop and validate a novel strategy for accelerating FPM image acquisition.
To enable high-speed, wide-field, high-resolution imaging for dynamic applications.

Main Methods:

Implementation of a wavelength multiplexing strategy within the FPM framework.
Exploration of spectral domain multiplexing for enhanced acquisition speed.
Development of a proof-of-concept system to demonstrate the feasibility of the proposed method.

Main Results:

The wavelength multiplexing strategy successfully accelerated the FPM acquisition process several-fold.
Experimental results validated the effectiveness of the spectral domain approach for high-speed FPM.
The strategy is compatible with existing FPM methodologies.

Conclusions:

The proposed wavelength multiplexing strategy is a viable approach to achieve high-speed FPM.
This method opens possibilities for high-speed gigapixel microscopy, expanding FPM's applicability to dynamic imaging.
The technique offers a significant advancement for real-time, high-resolution microscopic analysis.