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In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
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Multilineage communication regulates human liver bud development from pluripotency
J Gray Camp1, Keisuke Sekine2, Tobias Gerber1
1Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, Leipzig 04103, Germany.
Nature
|June 15, 2017
Summary
Three-dimensional liver organoids better model human liver development than 2D cultures. Vascular endothelial growth factor (VEGF) signaling promotes hepatoblast differentiation and organoid development.
Area of Science:
- Developmental biology
- Stem cell biology
- Organoid technology
Background:
- Two-dimensional differentiation fails to capture organogenesis complexity.
- Three-dimensional organoids offer better models but lineage interactions are unclear.
Purpose of the Study:
- To compare 2D and 3D liver development models.
- To investigate heterotypic interactions in liver organoids.
- To understand human liver development.
Main Methods:
- Single-cell RNA sequencing
- Three-dimensional liver bud organoid derivation
- Receptor-ligand pairing analysis
- High-throughput inhibitor assays
Main Results:
- Liver bud organoids mimic fetal liver development.
- Hepatoblasts in organoids show distinct lineage progression.
- VEGF signaling enhances endothelial networks and hepatoblast differentiation.
Conclusions:
- Three-dimensional organoids reveal insights into human liver development.
- Interlineage communication is crucial for organoid development.
- Organoid models can illuminate developmental processes.
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