Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Study on effect of coptidis rhizoma on red blood cells of normal mice and its antioxidant property].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2013
Same author

General framework to histogram-shifting-based reversible data hiding.

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society·2013
Same author

The prevalences of Neisseria gonorrhoeae and Chlamydia trachomatis infections among female sex workers in China.

BMC public health·2013
Same author

[Study on allocation rules of common nutrients in Scutellaria baicalensis in different phenological periods by ICP-OES].

Guang pu xue yu guang pu fen xi = Guang pu·2013
Same author

Sesterterpenoids.

Natural product reports·2013
Same author

14-3-3 sigma is a useful immunohistochemical marker for diagnosing ovarian granulosa cell tumors and steroid cell tumors.

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists·2013

Related Experiment Video

Updated: Feb 28, 2026

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
06:18

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause

Published on: August 13, 2019

13.0K

17β-Estradiol and progesterone decrease MDP induced NOD2 expression in bovine mammary epithelial cells.

Dan-Dan Xu1, Gang Wang1, Xian-Jing He1

  • 1College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.

Veterinary Immunology and Immunopathology
|June 16, 2017
PubMed
Summary
This summary is machine-generated.

Periparturient hormone changes in cows, specifically 17β-estradiol (E2) and progesterone (P4), decrease NOD2 expression in mammary cells. This down-regulation may increase susceptibility to mastitis.

Keywords:
17β-EstradiolBovine mammary epithelial cellNucleotide-binding oligomerization domain-like receptorProgesterone

More Related Videos

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
05:36

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

Published on: September 6, 2018

9.9K
A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary
07:03

A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary

Published on: September 19, 2025

671

Related Experiment Videos

Last Updated: Feb 28, 2026

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
06:18

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause

Published on: August 13, 2019

13.0K
A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
05:36

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

Published on: September 6, 2018

9.9K
A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary
07:03

A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary

Published on: September 19, 2025

671

Area of Science:

  • Veterinary immunology
  • Endocrinology
  • Innate immunity

Background:

  • Periparturient dairy cows experience significant neuroendocrine changes.
  • Hormonal fluctuations, including 17β-estradiol (E2) and progesterone (P4), can impact mammary gland immunity.
  • Mastitis susceptibility increases during the periparturient period.

Purpose of the Study:

  • To investigate the effects of E2 and P4 on NOD2 expression in bovine mammary epithelial cells (BMECs).
  • To understand the role of these hormones in regulating innate immune responses within the bovine mammary gland.

Main Methods:

  • Bovine mammary epithelial cells (BMECs) were isolated and treated with E2, P4, and muramyl dipeptide (MDP).
  • Quantitative real-time PCR (qRT-PCR) was used to measure mRNA levels of NOD2 and downstream inflammatory molecules (RIPK2, IL-1β, IL-6, IL-8, TNF-α).
  • Western blotting was employed to assess NOD2 protein expression.

Main Results:

  • Both E2 and P4 significantly decreased MDP-induced mRNA expression of NOD2 and associated inflammatory mediators.
  • E2 treatment also led to a reduction in MDP-induced NOD2 protein levels.
  • These findings indicate a suppressive effect of E2 and P4 on NOD2-mediated immune signaling.

Conclusions:

  • Down-regulation of NOD2 by E2 and P4 may contribute to increased mastitis susceptibility in periparturient dairy cows.
  • Understanding these hormonal influences on innate immunity is crucial for developing strategies to prevent mastitis.