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Related Experiment Video

Updated: Feb 28, 2026

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

Published on: June 9, 2011

Wild snakes harbor West Nile virus.

C R Dahlin¹, D F Hughes², W E Meshaka³

¹Department of Biology, University of Pittsburgh at Johnstown, Johnstown, PA 15904, United States.

One Health (Amsterdam, Netherlands)

|June 16, 2017

Summary

Wild snakes in Pennsylvania were found to carry West Nile virus (WNV). This research suggests snakes may play a role in WNV transmission, adding to our understanding of the virus

Keywords:

Flavirus RNA Reptile Snake Virus West Nile virus

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Last Updated: Feb 28, 2026

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

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Published on: June 9, 2011

Vector Competence Analyses on Aedes aegypti Mosquitoes using Zika Virus

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Published on: May 31, 2020

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Published on: August 31, 2022

Area of Science:

Veterinary Medicine
Epidemiology
Herpetology

Background:

West Nile virus (WNV) primarily cycles between birds and mosquitoes.
The role of reptiles, particularly snakes, in WNV eco-epidemiology is not well-established.
Understanding WNV reservoirs is crucial for disease management.

Purpose of the Study:

To investigate the presence of West Nile virus in a wild snake population in Pennsylvania.
To assess the potential role of snakes in WNV transmission dynamics.

Main Methods:

Oral sampling of six snake species in Pennsylvania during summer 2013.
Detection of WNV viral RNA using reverse transcription-polymerase chain reaction (RT-PCR).

Main Results:

West Nile virus RNA was detected in two out of 123 Eastern Garter Snakes (Thamnophis sirtalis sirtalis) sampled.
The prevalence of WNV in this snake population was 1.62%.

Conclusions:

Snakes, specifically Eastern Garter Snakes, can harbor West Nile virus.
These findings suggest snakes may contribute to the complex transmission cycle of WNV.
Further research is needed to fully elucidate the role of reptiles in WNV ecology.