HPLC-MS/MS method for troventol determination in human plasma and its application to biological samples

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Troventol (TRV) is a pharmaceutical agent requiring precise quantification in biological matrices.
• Existing methods may lack the sensitivity or specificity for low-level troventol detection.

Purpose of the Study:

• To develop and validate a sensitive HPLC-MS/MS method for determining troventol (TRV) levels in human plasma.
• To establish a reliable analytical tool for TRV quantification in biological samples.

Main Methods:

• Solid-phase extraction using Waters Oasis WCX cartridges.
• Chromatographic separation on a YMC-Pack SIL analytical column.
• Detection via HPLC-MS/MS with selected ion monitoring in positive ionization mode.

Main Results:

• The method achieved high sensitivity, determining troventol (TRV) at pmol/L levels.
• Good linearity was observed for troventol (TRV) calibration curves in the range of 35-500 pg/mL.
• The method was successfully applied to plasma samples from healthy subjects after TRV inhalation.

Conclusions:

• A novel, validated HPLC-MS/MS method enables accurate quantification of troventol (TRV) in human plasma.
• This method provides a valuable tool for pharmacokinetic research and clinical monitoring of troventol.