Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selfish bacteria take up polysaccharides under deep ocean pressure: insights from in situ and ex situ measurements.

ISME communications·2026
Same author

Opportunities and challenges for analytical chemists: organ-on-chip devices as new approach methods (NAMs) for identifying potential toxins.

The Analyst·2026
Same author

Syngas Production at Si Hybrid Photoelectrodes Modified with Re(I) and Mn(I) Tricarbonyl Phenanthroline Complexes Containing Reactive Aryl Azide Groups.

ACS applied materials & interfaces·2026
Same author

Potential-Controlled Deposition of Multilayer CO<sub>2</sub> Reduction Catalyst Films onto Silicon Photoelectrodes Demonstrates Thickness-Dependent Catalytic Rates.

ACS applied materials & interfaces·2025
Same author

Immobilizing a Lehn-Type Catalyst with Nitrocyclocondensation Chemistries: CO<sub>2</sub> Reduction on Silicon Hybrid Photoelectrodes.

ACS applied materials & interfaces·2025
Same author

An analytical screening platform to differentiate acute and prolonged exposures of per- and polyfluoroalkyl substances on invasive cellular phenotypes.

Toxicological sciences : an official journal of the Society of Toxicology·2025
Same journal

Spatially distributed carbon quantum dots in TiO<sub>2</sub> for photothermal-assisted hydrogen production from seawater.

Chemical communications (Cambridge, England)·2026
Same journal

Ultrasonication-assisted preparation of Li<sub>3</sub>PS<sub>4</sub> suspension for all-solid-state Li-ion batteries.

Chemical communications (Cambridge, England)·2026
Same journal

Dual-active-site engineering in cobalt-porphyrin porous hyper-crosslinked polymers enables synergistic catalysis for CO<sub>2</sub> cycloaddition with epoxides.

Chemical communications (Cambridge, England)·2026
Same journal

Optimizing nickel and cobalt-based water oxidation electrocatalysts <i>via</i> iron post-modification.

Chemical communications (Cambridge, England)·2026
Same journal

Facile preparation of a dual-readout metal-organic framework with aggregation-induced emission for highly sensitive detection of GST-α and Fe<sup>2</sup>.

Chemical communications (Cambridge, England)·2026
Same journal

Plasma-assisted ammonia synthesis utilizing water as a hydrogen source: progress, challenges, and prospects.

Chemical communications (Cambridge, England)·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

Alternative Strategy to Analyze In Vitro Cell Invasion of 3D Cultures
04:35

Alternative Strategy to Analyze In Vitro Cell Invasion of 3D Cultures

Published on: August 9, 2024

841

3D cellular invasion platforms: how do paper-based cultures stack up?

Rachael M Kenney1, C Chad Lloyd, Nathan A Whitman

  • 1Department of Chemistry, University of North Carolina at Chapel Hill, Kenan and Caudill Laboratories, 125 South Road, Chapel Hill, NC 27599-3290, USA. mlockett@unc.edu.

Chemical Communications (Cambridge, England)
|June 17, 2017
PubMed
Summary
This summary is machine-generated.

Paper-based cultures offer a novel 3D in vitro model for studying cancer invasion. These platforms mimic tumor microenvironments, enabling precise quantification of cellular invasion and metastasis.

More Related Videos

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting
05:34

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

Published on: November 20, 2015

34.2K
Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture
07:44

Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

Published on: June 23, 2020

12.6K

Related Experiment Videos

Last Updated: Feb 28, 2026

Alternative Strategy to Analyze In Vitro Cell Invasion of 3D Cultures
04:35

Alternative Strategy to Analyze In Vitro Cell Invasion of 3D Cultures

Published on: August 9, 2024

841
A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting
05:34

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

Published on: November 20, 2015

34.2K
Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture
07:44

Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

Published on: June 23, 2020

12.6K

Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Cell Biology

Background:

  • Cellular invasion is critical for metastasis, the primary cause of cancer mortality.
  • The tumor microenvironment presents chemical and mechanical stresses that drive invasion.
  • In vitro assays are essential for studying cancer progression, requiring tissue-like, controllable environments.

Purpose of the Study:

  • To review and compare four in vitro invasion platforms.
  • To highlight paper-based cultures as a promising 3D platform for invasion studies.
  • To demonstrate the capabilities of paper-based cultures in mimicking in vivo conditions.

Main Methods:

  • Overview of four distinct cellular invasion assay platforms.
  • Focus on paper-based cultures as an emerging 3D culture technology.
  • Utilizing paper-based cultures to generate tissue-like structures and quantify invasion.

Main Results:

  • Paper-based cultures support co-cultures and extracellular matrix components.
  • Experimentally controlled gradients can be established within paper-based cultures.
  • Readouts allow quantification and differentiation of cells based on invasiveness.

Conclusions:

  • Paper-based cultures provide an experimentally powerful platform for invasion studies.
  • They offer advantages over 2D cultures by providing a more representative in vivo-like environment.
  • This platform facilitates systematic study and quantification of cancer cell invasion.