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FtsZ Polymerization Assays: Simple Protocols and Considerations
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ZipA and FtsA* stabilize FtsZ-GDP miniring structures.

Yaodong Chen1,2, Haiyan Huang3, Masaki Osawa4

  • 1Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi Province, 710069, P.R. China. ydchen@nwu.edu.cn.

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ZipA stabilizes Escherichia coli

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Area of Science:

  • Microbiology
  • Cell Biology

Background:

  • The bacterial cytokinetic division ring, essential for cell division, involves FtsZ filaments anchored by FtsA and ZipA.
  • Previous studies proposed ZipA as a Z-ring stabilizer, enhancing FtsZ assembly and bundling, but this has been recently questioned.
  • FtsA mutants, like FtsA*, can function independently of ZipA, prompting a re-evaluation of ZipA's role.

Purpose of the Study:

  • To reinvestigate the interaction between FtsZ and ZipA in vitro.
  • To clarify the role of ZipA in stabilizing FtsZ structures during bacterial cytokinesis.

Main Methods:

  • In vitro biochemical assays were used to study the interaction of purified FtsZ with ZipA.
  • FtsZ assembly and filament structures were analyzed under various conditions, including the presence of ZipA and FtsA mutants.
  • The effect of ZipA on different conformations of FtsZ filaments (FtsZ-GTP and FtsZ-GDP) was examined.

Main Results:

  • ZipA stabilizes and bundles both straight FtsZ-GTP filaments and highly curved FtsZ-GDP miniring structures.
  • FtsA* demonstrated a similar stabilizing effect on FtsZ-GDP minirings, independent of ZipA.
  • These findings indicate ZipA's role extends beyond stabilizing straight filaments.

Conclusions:

  • ZipA stabilizes curved FtsZ-GDP miniring structures, suggesting a role in the constriction phase of cell division.
  • FtsA* may compensate for ZipA by stabilizing these miniring structures, highlighting alternative pathways in division.
  • Both ZipA and FtsA* likely contribute to bacterial cytokinesis by stabilizing the essential FtsZ miniring conformation.