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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
18.0K

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Related Experiment Video

Updated: Feb 28, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
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Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

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Nanotechnologies for In Vitro IgE Testing.

Iwan Märki1, Fabien Rebeaud2

  • 1Abionic SA, Biopôle, sect. Esplanade SE-A, Route de la Corniche 3, CH-1066, Epalinges, Switzerland. iwan.maerki@abionic.com.

Current Allergy and Asthma Reports
|June 18, 2017
PubMed
Summary

Nanotechnology advancements are improving immunoglobulin E (IgE) antibody testing for allergies. These innovations offer faster, more sensitive, and efficient diagnostic methods for allergy experts.

Keywords:
Allergy diagnosisIgE blood testNanofluidicsNanoparticlesNanotechnology

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Area of Science:

  • Biotechnology
  • Nanomedicine
  • Analytical Chemistry

Background:

  • Immunoglobulin E (IgE) antibody testing is crucial for diagnosing IgE-mediated hypersensitivity.
  • Current diagnostic methods for allergies require optimization for efficiency and sensitivity.

Purpose of the Study:

  • To review recent advancements in nanotechnology-based IgE antibody assays.
  • To explore the transition of these assays from laboratory concepts to clinical applications.

Main Methods:

  • Application of nanoparticles as supports for analyte capture and signal enhancement.
  • Utilization of nanofluidics to accelerate molecular interactions and reduce assay times.

Main Results:

  • Nanotechnologies enable novel in vitro IgE testing methods.
  • Advantages include reduced sample volume, shorter assay duration, and simplified procedures.
  • Enhanced sensitivity without compromising test precision and accuracy.

Conclusions:

  • Nanotechnology offers significant improvements for IgE testing in allergy diagnostics.
  • Overcoming challenges is key to integrating these advanced assays into routine clinical practice.