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Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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Related Experiment Video

Updated: Feb 28, 2026

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
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Controlled release of basic fibroblast growth factor for angiogenesis using acoustically-responsive scaffolds.

Alexander Moncion1, Melissa Lin2, Eric G O'Neill2

  • 1Applied Physics Program, University of Michigan, Ann Arbor, MI, USA; Department of Radiology, University of Michigan Health System, Ann Arbor, MI, USA.

Biomaterials
|June 19, 2017
PubMed
Summary

Acoustically-responsive scaffolds (ARSs) enable controlled release of basic fibroblast growth factor (bFGF) using ultrasound. This technology enhances therapeutic angiogenesis, improving blood vessel formation and perfusion in vivo.

Keywords:
Acoustic droplet vaporizationAngiogenesisBasic fibroblast growth factorControlled releasePerfluorocarbonUltrasound

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Vascular Biology

Background:

  • Clinical translation of pro-angiogenic growth factors is hindered by safety and efficacy concerns.
  • Spatiotemporally-controlled delivery systems are crucial for recapitulating endogenous signaling and improving growth factor therapy.
  • Acoustically-responsive scaffolds (ARSs) offer a novel approach for on-demand, non-invasive growth factor release.

Purpose of the Study:

  • To investigate the in vitro and in vivo release of basic fibroblast growth factor (bFGF) from ARSs.
  • To characterize the bioactivity of released bFGF.
  • To evaluate the therapeutic potential of ARS-mediated bFGF delivery for angiogenesis.

Main Methods:

  • ARSs were fabricated by doping fibrin scaffolds with bFGF-encapsulated, sonosensitive emulsions using microfluidics.
  • Controlled release of bFGF was achieved using focused, 2.5 MHz ultrasound (US) at specific pressure thresholds.
  • In vitro release kinetics and bioactivity assays were performed, followed by subcutaneous implantation in mice to assess in vivo efficacy.

Main Results:

  • Ultrasound exposure above 2.2 ± 0.2 MPa peak rarefactional pressure triggered controlled bFGF release, with a 12.6-fold increase in vitro.
  • Released bFGF retained its bioactivity.
  • In vivo, US-treated ARSs showed significantly increased perfusion (3.3-fold) and blood vessel density (1.7-fold) compared to controls.
  • Scaffold degradation remained unaffected by ultrasound treatment.

Conclusions:

  • ARSs provide a viable platform for non-invasive, on-demand delivery of therapeutic growth factors like bFGF.
  • This technology demonstrates significant potential for enhancing therapeutic angiogenesis and treating vascular diseases.
  • ARSs are valuable tools for both fundamental research and applied studies in regenerative medicine.