Profiling of Vitis vinifera L. canes (poly)phenolic compounds using comprehensive two-dimensional liquid...

Area of Science:

• Agricultural Science
• Analytical Chemistry
• Biochemistry

Background:

• Grapevine canes, a byproduct of pruning, contain abundant bioactive (poly)phenolic compounds.
• Accurate chemical characterization is crucial for designing effective valorization strategies.
• Previous characterization methods struggled with the chemical variability of these compounds.

Purpose of the Study:

• To develop a comprehensive two-dimensional liquid chromatography (LC×LC) method for profiling (poly)phenolic compounds in grapevine canes.
• To compare different LC×LC setups to identify the optimal configuration for analysis.
• To characterize the diverse (poly)phenolic composition of grapevine canes from multiple varieties.

Main Methods:

• Development and optimization of a two-dimensional liquid chromatography (LC×LC) system.
• Testing and comparison of three different LC×LC column combinations.
• Utilizing diode array detection (DAD) and mass spectrometry (MS) for component identification.

Main Results:

• The optimal LC×LC setup combined diol and C18 columns, achieving a complete (poly)phenolic profile in approximately 80 minutes.
• A total of 81 different (poly)phenolic compounds were detected and tentatively assigned, including stilbenoids, procyanidins, and prodelphinidins.
• The method demonstrated high separation capabilities, with an effective peak capacity of 842 and orthogonality of 78%.

Conclusions:

• The developed LC×LC method provides a powerful tool for the comprehensive characterization of (poly)phenolic compounds in grapevine canes.
• Grapevine canes represent an underexploited natural source of valuable bioactive compounds.
• These findings support the potential application of grapevine cane extracts in various industries.