Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Biocompatibility of silk fibroin nanofibers scaffold with olfactory ensheathing cells].

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery·2009
Same author

[Association of tryptophan hydroxylase gene A218C and serotonin transporter gene polymorphism with essential hypertension in Chinese northern Han population].

Zhonghua xin xue guan bing za zhi·2009
Same author

An FES cycling control system based on CPG.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2009
Same author

One example on how colloidal nano- and microparticles could contribute to medicine.

Nanomedicine (London, England)·2009
Same author

[Mutational analysis of Meq, RLORF4, RLORF12 and 132bpr genes of epidemic Marek's disease virus strains highly passaged on chicken embryo fibroblast].

Bing du xue bao = Chinese journal of virology·2009
Same author

Dietary fish oil n-3 polyunsaturated fatty acids and alpha-linolenic acid differently affect brain accretion of docosahexaenoic acid and expression of desaturases and sterol regulatory element-binding protein 1 in mice.

The Journal of nutritional biochemistry·2009

Related Experiment Video

Updated: Feb 28, 2026

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
09:09

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

Published on: November 16, 2016

8.4K

A high-throughput dye-reducing photometric assay for evaluating microbial exoelectrogenic ability.

Xiang Xiao1, Qiu-Yue Liu2, Ting-Ting Li2

  • 1School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China; CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei 230026, China.

Bioresource Technology
|June 21, 2017
PubMed
Summary

Researchers developed a simple photometric assay to measure exoelectrogenic bacteria (EEB) ability by monitoring dye decolorization. This method efficiently evaluates EEB exoelectrogenic capacity for bioremediation and bioenergy applications.

Keywords:
Dye decolorizationExoelectrogenic abilityExoelectrogenic bacteriaHigh-throughputPhotometric sensor

More Related Videos

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
09:00

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

10.8K
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
20:28

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

14.7K

Related Experiment Videos

Last Updated: Feb 28, 2026

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
09:09

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

Published on: November 16, 2016

8.4K
Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
09:00

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

10.8K
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
20:28

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

14.7K

Area of Science:

  • Microbiology
  • Environmental Science
  • Biotechnology

Background:

  • Exoelectrogenic bacteria (EEB) facilitate electron transfer to external acceptors, offering potential in environmental bioremediation and bioenergy.
  • Effective methods for assessing EEB exoelectrogenic capabilities are crucial for their practical application.

Purpose of the Study:

  • To develop a simple, efficient photometric assay for evaluating the exoelectrogenic ability of EEB.
  • To utilize extracellular dye reduction as a quantifiable measure of microbial exoelectrogenesis.

Main Methods:

  • A plate-based photometric assay was designed using high polar dyes, specifically methyl orange, as electron acceptors.
  • The assay monitored extracellular dye decolorization under anaerobic conditions to assess EEB exoelectrogenic activity.
  • The method was validated using Shewanella oneidensis MR-1 wild-type and Mtr mutants, and correlated with microbial fuel cell mixed cultures.

Main Results:

  • The developed photometric assay effectively measured the exoelectrogenic ability of various EEB strains.
  • Methyl orange was confirmed as a suitable probe for evaluating EEB exoelectrogenesis.
  • The assay demonstrated accurate assessment of Shewanella oneidensis MR-1 strains and correlated well with microbial fuel cell performance.

Conclusions:

  • This novel photometric assay provides a rapid and efficient means to evaluate microbial exoelectrogenic ability.
  • The method is applicable to pure cultures and mixed microbial communities, facilitating research in bioremediation and bioenergy.
  • This assay supports the practical implementation and advancement of EEB-based technologies.