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The manganese efflux system MntE contributes to the virulence of Streptococcus suis serotype 2

Jiali Xu1, Chengkun Zheng1, Manman Cao1

  • 1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China.

Microbial Pathogenesis
|June 21, 2017
PubMed

Insights

The manganese efflux system MntE is crucial for Streptococcus suis serotype 2 survival under high manganese conditions and oxidative stress. MntE also plays a significant role in the bacterium

Area of Science:

  • Microbiology
  • Bacterial Physiology
  • Molecular Biology

Background:

  • Manganese is an essential bacterial micronutrient, vital for cellular functions.
  • Excess manganese is toxic to bacteria, necessitating regulatory mechanisms.
  • Manganese efflux systems control intracellular manganese levels in some bacteria.

Purpose of the Study:

  • Identify and characterize the manganese efflux system in Streptococcus suis serotype 2.
  • Investigate the role of the MntE protein in manganese homeostasis and bacterial virulence.

Main Methods:

  • Construction of mntE deletion mutant (ΔmntE) and complementation strain (CΔmntE) in S. suis 2.
  • Growth curve analysis under varying manganese concentrations.
  • Assessment of sensitivity to oxidative stress (diamide).
  • Competitive-infection assays in a murine model.

Main Results:

  • ΔmntE mutant showed normal growth but was defective in high manganese media.
  • The mutant exhibited increased sensitivity to oxidative stress.
  • MntE was demonstrated to be essential for S. suis 2 virulence in vivo.

Conclusions:

  • Manganese homeostasis, regulated by the MntE efflux system, is critical for S. suis 2 pathogenesis.
  • MntE contributes to bacterial survival against manganese toxicity and oxidative stress.
  • MntE is a key factor in the virulence of Streptococcus suis serotype 2.
Keywords:
Manganese efflux systemMntEStreptococcus suisVirulence

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