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Updated: Feb 28, 2026

Sample Preparation for Mass Cytometry Analysis
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Rapid monoisotopic cisplatin based barcoding for multiplexed mass cytometry.

Ryan L McCarthy1, Duncan H Mak2, Jared K Burks2

  • 1Department of Epigenetics and Molecular Carcinogenesis, Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. rlmccarthy@mdanderson.org.

Scientific Reports
|June 21, 2017
PubMed
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This summary is machine-generated.

Mass cytometry enables detailed cell analysis but suffers from sample variability. A new, rapid cisplatin barcoding method reduces artifacts, improving data accuracy and throughput for complex biological studies.

Area of Science:

  • Single-cell biology
  • Proteomics
  • Biotechnology

Background:

  • Mass cytometry offers high-dimensional proteomic analysis of heterogeneous cell populations.
  • Sample-to-sample variability in mass cytometry can lead to data artifacts and incorrect conclusions.
  • Current barcoding methods are time-consuming, reduce parameter counts, or use costly reagents.

Purpose of the Study:

  • To develop a rapid, cost-effective, and efficient cell barcoding technique for mass cytometry.
  • To mitigate sample-to-sample variability and improve data consistency.
  • To enhance the multiplexing capacity of mass cytometry experiments.

Main Methods:

  • Utilized monoisotopic cisplatin for cell barcoding without requiring cell permeabilization.
  • Developed a 10-minute barcoding protocol.

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  • Integrated cisplatin barcoding with existing multiplexing techniques.

    • Main Results:

    • Successfully implemented a rapid cell barcoding method using cisplatin.
    • The method eliminates the need for cell permeabilization and is completed in 10 minutes.
    • Cisplatin barcoding can be combined with other techniques to significantly increase sample multiplexing.

    Conclusions:

    • Cisplatin-based cell barcoding is a fast, efficient, and versatile solution for mass cytometry.
    • This approach reduces experimental artifacts, enhances throughput, and improves data reliability.
    • The method offers a cost-effective alternative to existing barcoding strategies, advancing single-cell proteomic analysis.