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Ionizing radiation expands a p90RSK-activated patrolling monocyte subset: modulation by colchicine
Masaki Imanishi1, Venkata S K Samanthapudi1, Nhat-Tu Le2
1Department of Cardiology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States.
Colchicine inhibits radiation-induced expansion of specific monocytes by modulating p90RSK signaling, potentially reducing vascular inflammation and cardiovascular risk in cancer survivors.
Area of Science:
- Immunology
- Molecular Biology
- Radiation Oncology
Background:
- Ionizing radiation (IR) causes vascular injury and immune cell dysfunction.
- Mechanisms involving clonal hematopoiesis proteins and stress pathways in monocytes are unclear.
- The role of DNMT3A and TET2 in radiation-induced monocyte changes requires further investigation.
Purpose of the Study:
- To investigate the effects of IR and colchicine on monocyte subsets.
- To elucidate the role of clonal hematopoiesis-associated proteins (DNMT3A, TET2) and p90RSK signaling in IR-exposed monocytes.
- To determine if colchicine can mitigate IR-induced immune alterations.
Main Methods:
- Peripheral blood mononuclear cells (PBMCs) were treated with colchicine and exposed to 2 Gy IR.
- Mass cytometry (CyTOF) was used with a comprehensive antibody panel.
- Data analysis involved Astrolabe platform for immune subset annotation.
Main Results:
- IR did not alter overall immune cell frequency, but colchicine increased myeloid and B cells.
- IR enhanced p90RSK activation in CD14-CD16+ monocytes, reducing proliferation and inflammatory markers.
- IR decreased DNMT3A and TET2 expression, correlating with elevated p90RSK in CD14-CD16+CD68hi monocytes, which expanded post-IR.
- Colchicine suppressed this expansion without restoring DNMT3A/TET2, indicating selective p90RSK pathway modulation.
Conclusions:
- Colchicine selectively inhibits the expansion of CD14-CD16+CD68hi monocytes, characterized by reduced CHIP proteins and heightened p90RSK.
- This suggests colchicine modulates radiation-responsive monocyte signaling.
- Findings support a hypothesis that colchicine may mitigate radiation-induced vascular inflammation and cardiovascular risk.
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