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Gram-Scale Production of Ginsenoside F1 Using a Recombinant Bacterial β-Glucosidase
Dong-Shan An1, Chang-Hao Cui2, Muhammad Zubair Siddiqi3,4
1Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
Journal of Microbiology and Biotechnology
|June 22, 2017
Summary
Researchers produced large quantities of rare ginsenoside F1 using an enzyme. This novel biotransformation method offers a scalable way to obtain high-purity ginsenoside F1 for potential applications.
Area of Science:
- Biotechnology
- Enzymology
- Natural Products Chemistry
Background:
- Ginsenoside F1 is a rare but valuable compound.
- Previous methods for obtaining ginsenoside F1 were limited in scale.
Purpose of the Study:
- To develop a scalable method for producing ginsenoside F1.
- To optimize enzyme-mediated biotransformation for ginsenoside F1 synthesis.
Main Methods:
- Enzyme-mediated biotransformation using recombinant beta-glucosidase (BgpA) from Terrabacter ginsenosidimutans.
- Optimization of substrate, enzyme, and NaCl concentrations.
- Octadecylsilyl silica gel column chromatography for purification.
Main Results:
- Gram-scale quantities of ginsenoside F1 were produced.
- 9.6 g of ginsenoside F1 was obtained from 60 g of substrate mixture with 95% purity.
- Optimized conditions included 200 mM NaCl, 25 mg/ml substrate, and 20 mg/ml enzyme.
Conclusions:
- This study reports the first gram-scale production of ginsenoside F1.
- Recombinant enzyme-mediated biotransformation is an effective method for scalable ginsenoside F1 synthesis.
