Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Niche01:26

Stem Cell Niche

6.5K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Subtype-specific bone mineralization defects and early treatment amelioration in murine models of autosomal recessive osteopetrosis revealed by Raman spectroscopy.

Bone·2026
Same author

Hydroxy-Group Topology as a Molecular Trigger Between Antioxidant and Photosensitizing Properties in Dihydroxynaphthalenes.

ACS omega·2026
Same author

Advancing mechanobiology from single molecules to complex cellular systems.

Nature nanotechnology·2026
Same author

Nonequilibrium dynamics of high energy transitions in monolayer WSe<sub>2</sub>.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same author

Ultrafast Excited-State Dynamics of Dithienyltetrazine-Based Donor-Acceptor Copolymers.

The journal of physical chemistry. B·2026
Same author

Integration of 2D Materials in Radial van der Waals Heterostructure Metasurfaces.

ACS nano·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions
07:38

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Published on: June 7, 2024

2.4K

3D Stem Cell Niche Engineering via Two-Photon Laser Polymerization.

Michele M Nava1, Tommaso Zandrini2, Giulio Cerullo2

  • 1Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, 32 piazza Leonardo da Vinci, Milano, Italy. michele.nava@polimi.it.

Methods in Molecular Biology (Clifton, N.J.)
|June 22, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel "nichoid" culture substrate using laser two-photon polymerization to precisely control microarchitecture for studying stem cell behavior. This technology advances the creation of synthetic niches for stem cell research.

Keywords:
Engineered nicheMechanobiologyNichoidTwo-photon polymerization

More Related Videos

Control of Cell Geometry through Infrared Laser Assisted Micropatterning
11:04

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

3.9K
Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates
08:07

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

9.0K

Related Experiment Videos

Last Updated: Feb 28, 2026

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions
07:38

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Published on: June 7, 2024

2.4K
Control of Cell Geometry through Infrared Laser Assisted Micropatterning
11:04

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

3.9K
Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates
08:07

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

9.0K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Nanotechnology

Background:

  • Mimicking the native cell-extracellular matrix (ECM) interaction is key to modulating stem cell behavior in culture.
  • Synthetic niches are artificial microenvironments that replicate aspects of stem cell-ECM interactions, including biochemical and biophysical factors.
  • Existing scaffold fabrication methods often lack submicrometer control over substrate geometry, which is crucial for stem cell fate.

Purpose of the Study:

  • To develop an innovative culture substrate for studying mesenchymal stem cell mechanobiology.
  • To overcome limitations in current scaffold fabrication regarding precise control of microarchitecture.
  • To create a novel synthetic niche with submicrometer geometrical control.

Main Methods:

  • Utilized laser two-photon polymerization (2PP), a mask-less direct laser writing technique.
  • Fabricated a novel culture substrate, termed "nichoid," using a hybrid organic-inorganic photoresist (SZ2080).
  • Employed 2PP to achieve precise, submicrometer control over the microarchitecture of the synthetic niche.

Main Results:

  • Successfully developed the "nichoid" substrate with controlled microarchitectural features.
  • Demonstrated the capability of laser 2PP to create complex 3D microstructures for cell culture.
  • Established a platform for investigating mesenchymal stem cell mechanobiology with high geometrical precision.

Conclusions:

  • The developed "nichoid" substrate offers unprecedented control over microenvironmental geometry for stem cell research.
  • Laser two-photon polymerization is a powerful tool for fabricating advanced synthetic niches.
  • This technology provides a new avenue for understanding stem cell mechanobiology and guiding cell fate determination.