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Hydrogen Peroxide Sensing Based on Inner Surfaces Modification of Solid-State Nanopore.

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This study presents a novel solid-state nanopore biosensor for detecting hydrogen peroxide (H2O2). The reversible sensor utilizes immobilized enzymes for label-free, real-time molecular detection.

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Molecular detection is crucial in various scientific fields.
  • Solid-state nanopore technology offers a promising avenue for high-throughput, low-cost molecular analysis.
  • Hydrogen peroxide (H2O2) is a significant molecule involved in redox reactions, necessitating reliable detection methods.

Purpose of the Study:

  • To fabricate and characterize a solid-state nanopore platform for label-free detection of hydrogen peroxide (H2O2).
  • To immobilize horseradish peroxidase (HRP) within the nanopore for enzymatic redox reactions.
  • To monitor single molecular translocation events in real-time.

Main Methods:

  • Fabrication of silicon nitride (Si3N4) nanopores (~50 nm diameter) using a focused Ga ion beam.
  • Surface modification of nanopores with horseradish peroxidase (HRP) via carbodiimide coupling chemistry.
  • Real-time monitoring of aggregated ABTS•+ molecular translocation events.

Main Results:

  • Successful fabrication of functionalized silicon nitride nanopores.
  • Demonstrated ability of immobilized HRP to induce redox reactions within the nanopore.
  • Observed and investigated real-time single aggregated ABTS•+ molecular translocation events.
  • The developed biosensor showed reversibility and reusability for H2O2 detection.

Conclusions:

  • A novel solid-state nanopore biosensor for label-free H2O2 detection has been successfully developed.
  • The sensor leverages enzymatic activity and single-molecule translocation for sensitive detection.
  • The reversible and reusable nature of the biosensor highlights its potential for practical applications.