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Somatostatin receptors.

C B Srikant, Y C Patel

    Advances in Experimental Medicine and Biology
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a superior radioligand for quantifying somatostatin-14 (S-14) receptors, revealing their distribution in the central nervous system and peripheral tissues. Pancreatic acinar cells show the highest affinity for S-14 binding.

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    Area of Science:

    • Neuroscience
    • Endocrinology
    • Pharmacology

    Background:

    • Biological actions of tetradecapeptide somatostatin (somatostatin-14, S-14) are receptor-mediated.
    • Previous radioligands like [125I-Tyr1] S-14 showed high non-specific binding.
    • Need for improved methods to study S-14 receptors in various tissues.

    Purpose of the Study:

    • To develop a more suitable radioligand for S-14 receptor quantification.
    • To map the distribution of S-14 receptors in the central nervous system (CNS) and peripheral tissues.
    • To characterize the binding affinity of S-14 receptors in different tissues.

    Main Methods:

    • Utilized [125I-Tyr11] S-14, a novel radioligand with reduced non-specific binding.
    • Quantified S-14 receptor concentrations in various CNS regions (cerebral cortex, thalamus, hypothalamus, etc.).

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  • Characterized S-14 membrane receptors in peripheral tissues (pituitary, adrenal cortex, pancreatic acini).

    • Main Results:

    • [125I-Tyr11] S-14 demonstrated significantly lower non-specific binding compared to [125I-Tyr1] S-14.
    • Highest S-14 receptor concentrations were observed in the cerebral cortex, followed by thalamus and hypothalamus.
    • Peripheral tissues like pituitary, adrenal cortex, and pancreatic acini possess S-14 receptors.
    • Pancreatic acinar cells exhibited the highest affinity for S-14 binding among all tissues studied.

    Conclusions:

    • [125I-Tyr11] S-14 is a more suitable radioligand for S-14 receptor studies.
    • S-14 receptors are widely distributed in the CNS and peripheral organs.
    • Significant variations in S-14 receptor binding affinity exist across different tissues, with pancreatic acini being notable.