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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.7K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Photoluminescence: Fluorescence and Phosphorescence01:23

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Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
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Plasmon-enhanced fluorescence spectroscopy.

Jian-Feng Li1, Chao-Yu Li, Ricardo F Aroca

  • 1MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, iChEM, College of Chemistry and Chemical Engineering, Department of Physics, Research Institute for Biomimetics and Soft Matter, Xiamen University, Xiamen 361005, China. Li@xmu.edu.cn.

Chemical Society Reviews
|June 23, 2017
PubMed
Summary
This summary is machine-generated.

Plasmon-enhanced fluorescence (PEF) significantly boosts detection sensitivity and imaging resolution, even for weak emitters. This review highlights PEF

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Area of Science:

  • Optics and Photonics
  • Spectroscopy
  • Nanotechnology

Background:

  • Fluorescence spectroscopy offers ultra-high sensitivity for single-molecule detection.
  • Plasmon-enhanced fluorescence (PEF) improves emission, reduces lifetimes, and enables super-resolution imaging.
  • PEF allows imaging beyond the diffraction limit and incorporates weak quantum emitters.

Purpose of the Study:

  • To review recent advancements in plasmon-enhanced fluorescence (PEF).
  • To emphasize the mechanism of plasmon enhancement and substrate preparation.
  • To discuss advanced applications and future outlook of PEF.

Main Methods:

  • Coupling fluorophores with localized surface plasmons on nanoparticles.
  • Utilizing shell-isolated nanostructure-enhanced fluorescence for surface analysis.
  • Investigating local field enhancement for improved molecular emission.

Main Results:

  • PEF enhances molecular emission brightness and detection sensitivity.
  • PEF enables imaging with resolutions surpassing the diffraction limit.
  • PEF facilitates the development of photostable probes by combining metal nanostructures and quantum emitters.

Conclusions:

  • PEF is a powerful technique for sensitive detection and advanced imaging.
  • Continued research in PEF promises high time- and spatially resolved properties.
  • PEF expands the capabilities of fluorescence spectroscopy for various applications.