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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Animal Mitochondrial Genetics

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Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

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Related Experiment Video

Updated: Feb 27, 2026

Gene Editing of Primary Rhesus Macaque B Cells

Gene Editing of Primary Rhesus Macaque B Cells

Published on: February 10, 2023

Genome Editing of Monkey.

Zhen Liu¹, Yijun Cai¹, Qiang Sun^2,3

¹Nonhuman Primate Research (SuZhou) Facility, Institute of Neuroscience, Chinese Academy of Sciences (CAS) Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, CAS, Shanghai, 200031, China.

Methods in Molecular Biology (Clifton, N.J.)

|June 24, 2017

Summary

This summary is machine-generated.

We generated gene-modified monkeys using lentivirus and gene-editing tools like CRISPR-cas9. These advanced models offer high efficiency for studying gene function in biomedical and neuroscience research.

Keywords:

Gene-modified monkeys Knockout Lentivirus Programmable nucleases Transgenic

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Last Updated: Feb 27, 2026

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Published on: February 10, 2023

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Dissecting the Non-human Primate Brain in Stereotaxic Space

Dissecting the Non-human Primate Brain in Stereotaxic Space

Published on: July 16, 2009

Area of Science:

Biomedical Research
Neuroscience
Genetics

Background:

Gene-modified non-human primates are crucial for advancing biomedical and neuroscience research.
Existing methods for genetic modification in monkeys have limitations in efficiency and precision.

Purpose of the Study:

To describe the efficient generation of gene-modified monkeys using lentivirus and programmable nucleases.
To create transgenic and knock-out monkey models for studying specific gene functions.

Main Methods:

Utilized lentivirus for gene delivery.
Employed programmable nucleases, including TALEN and CRISPR-cas9, for site-specific gene editing.
Generated both gene-added (transgenic) and gene-deleted (knock-out) monkey models.

Main Results:

Achieved high efficiency in generating gene-modified monkeys.
Successfully produced transgenic monkeys with gene gain-of-function.
Successfully produced knock-out monkeys with gene loss-of-function.

Conclusions:

Lentivirus and programmable nucleases provide an efficient platform for generating gene-modified monkeys.
These models are valuable tools for in vivo research in genetics, neuroscience, and broader biomedical fields.