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Related Experiment Video

Updated: Feb 27, 2026

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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Genome Editing of C. elegans.

Takuma Sugi1

  • 1Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu, Shiga, 520-2192, Japan. tsugi@belle.shiga-med.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|June 24, 2017
PubMed
Summary

Caenorhabditis elegans is a model organism for genetic studies. This work details a common genome editing protocol for this nematode, enhancing genetic analysis capabilities.

Area of Science:

  • Genetics
  • Developmental Biology
  • Molecular Biology

Background:

  • Caenorhabditis elegans is a widely used model organism for genetic research due to its rapid life cycle, transparency, and well-annotated genome.
  • Its simple morphology and genetic tractability make it ideal for studying complex biological processes.
  • Genome editing technologies are increasingly vital for advancing genetic investigations in C. elegans.

Purpose of the Study:

  • To introduce a frequently employed genome editing protocol for Caenorhabditis elegans.
  • To facilitate advanced genetic analyses in this model organism.

Main Methods:

  • The study focuses on a specific, commonly used genome editing protocol.
  • Detailed procedural steps are outlined for researchers.
Keywords:
C. elegansCRISPR/Cas9Homology-dependent repair

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Main Results:

  • The protocol is presented as a practical method for C. elegans genome editing.
  • Successful application of the protocol enables precise genetic modifications.

Conclusions:

  • Genome editing in C. elegans is a powerful tool for biological research.
  • The presented protocol offers a reliable method for genetic manipulation in this model system.