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Published on: December 27, 2017
Idiopathic thrombocytopenic purpura: pathogenesis and potential therapeutic approach
Xiaohong Zhao1, Xiaomei Qi1, Chen Wang1
1Department of Hematology, Wuxi Second People's Hospital, Nanjing Medical University, Wuxi, China.
Background:
This study sought to clarify the pathogenesis of idiopathic thrombocytopenic purpura (ITP) and make preliminary investigations regarding a therapeutic approach.
Methods:
Enzyme-linked immunosorbent assay was used to establish and analyze the standard curve for interferon (IFN) γ, interleukin (IL) 4, and IL-17 in order to determine a measurement method for these cytokines. Subsequently, cellular levels of IFN-γ, IL-4, and IL-17 in the peripheral blood of patients in the treatment group (traditional Chinese medicine) was compared with those in the control group (Western-style care). An ITP mice model was also established and treated with different medications.
Results:
Th1/Th2 cell quantities in ITP patients were significantly higher than in healthy individuals (P<0.05). Furthermore, IL-17 secreted by Th17 cells was significantly higher in ITP patients than healthy individuals (P<0.01). A combination of traditional Chinese medicine and Western-style care yielded the best treatment effect for ITP mice, followed by Western medicine alone and then Chinese medicine alone.
Conclusions:
The experimental results suggested that Th17 cells may be more related to the pathogenesis of ITP, and that application of Western-style care supplemented by traditional Chinese medicine may yield a more optimal treatment for ITP.
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