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Evaluation of a Rapid Method for Screening Heat Stress Tolerance Using Three Korean Wheat (Triticum aestivum L.)
Hai An Truong1, Chan Young Jeong1,2, Won Je Lee1
1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Anam-dong 5-ga, Seongbuk-gu, Seoul 02841, Republic of Korea.
Abstract:
Thermotolerance in plants is a topic of concern given the current trends in global warming. Here, we aimed to develop a rapid and reproducible screening method for selection of heat stress-tolerant wheat varieties to expedite the breeding process. We tested the robustness of the screen in three Korean wheat cultivars, "BackJung", "KeumKang", and "ChoKyeong". We showed that 4-day-old seedlings of "KeumKang" had the highest survival rates after a 45 °C treatment for 20 h. Moreover, the ability to retain chlorophyll and antioxidant activity was also highest in "KeumKang". The increase in malondialdehyde content in "ChoKyeong" indicated that this cultivar showed the greatest damage after heat stress. Collectively, our results showed that "KeumKang" is the most heat-tolerant cultivar of the three examined. In conclusion, the most reliable and rapid screening method in our investigation was survival rate examined at lethal temperature.
