Related Experiment Video

Updated: Feb 27, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

9.0K

DNA-methylation-mediated repression of miR-766-3p promotes cell proliferation via targeting SF2 expression in renal

Chen Chen1, Sheng Xue2,3, Jin Zhang4

  • 1Department of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, Shanghai, 200072, China.

International Journal of Cancer
|June 29, 2017
PubMed

Insights

Epigenetic silencing of miR-766-3p via methylation is observed in renal cell carcinoma (RCC). Restoring miR-766-3p inhibits RCC progression by targeting SF2, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Aberrant microRNA (miRNA) expression is implicated in human malignancies.
  • Identifying and validating altered miRNAs is crucial for understanding cancer.
  • Renal cell carcinoma (RCC) pathogenesis requires further elucidation.

Purpose of the Study:

  • To identify novel epigenetic regulation of miR-766-3p in RCC.
  • To investigate the biological function and clinical significance of miR-766-3p in RCC.
  • To explore the therapeutic potential of targeting the miR-766-3p/SF2 axis in RCC.

Main Methods:

  • Bisulfate analysis to assess DNA methylation of the miR-766-3p promoter.
  • Correlation analysis between miR-766-3p expression levels and clinical parameters in RCC patients.
  • In vitro experiments to determine the effect of miR-766-3p on cell-cycle progression and SF2 expression.
  • Analysis of SF2 protein levels and its association with patient survival in RCC cohorts.

Main Results:

  • The promoter of miR-766-3p is significantly methylated in RCC tissues.
  • Downregulation of miR-766-3p correlates with advanced clinical stage and poor prognosis in RCC patients.
  • Upregulation of miR-766-3p inhibits cell-cycle progression by targeting SF2 and the SF2/P-AKT/P-ERK pathway.
  • High SF2 expression, a novel oncoprotein in RCC, is associated with poor survival.

Conclusions:

  • Epigenetic silencing of miR-766-3p by promoter methylation is a key event in RCC.
  • The miR-766-3p/SF2 axis plays a critical role in RCC progression.
  • Targeting the miR-766-3p/SF2 axis presents a promising therapeutic strategy for RCC.
Keywords:
DNA-methylationSF2miR-766-3pproliferationrenal cell carcinoma

More Related Videos

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.2K
Methyl-binding DNA capture Sequencing for Patient Tissues08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

9.1K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.0K
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K