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A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

Revolving around constriction by ESCRT-III.

Henri G Franquelim¹, Petra Schwille¹

¹Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.

Nature Cell Biology

|June 30, 2017

Summary

The endosomal sorting complex required for transport (ESCRT)-III machinery forms spiraling filaments critical for cell division. Their dynamic assembly and disassembly drive membrane abscission during cytokinesis.

Area of Science:

Cell Biology
Molecular Biology
Biophysics

Background:

The endosomal sorting complex required for transport (ESCRT)-III machinery is essential for membrane abscission.
The precise mechanism of ESCRT-III-mediated membrane constriction is not fully understood.

Purpose of the Study:

To investigate the dynamic assembly and disassembly of the ESCRT-III machinery.
To elucidate the role of ESCRT-III filament dynamics in membrane abscission during cytokinesis.

Main Methods:

In vitro reconstitution assays of ESCRT-III filament dynamics.
In vivo imaging of ESCRT-III machinery during cytokinesis.

Main Results:

Observed dynamic assembly and disassembly of ESCRT-III spiraling filaments.

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Demonstrated a correlation between filament turnover and membrane constriction.

Conclusions:

The turnover of ESCRT-III filaments is a critical factor in membrane abscission.
ESCRT-III filament dynamics provide a mechanistic basis for membrane constriction during cell division.