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Updated: Feb 27, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Sonication-Based Basic Protocol for Liposome Synthesis.

Roberto Mendez¹, Santanu Banerjee²

¹Division of Surgical Oncology, Department of Surgery, Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, 1550 NW 10th Ave., Pap 303, Miami, FL, 33136, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 30, 2017

Summary

This summary is machine-generated.

This chapter introduces liposomes, versatile nanoscale vesicles used for drug delivery. It provides a basic protocol for preparing and qualifying liposomes for scientific research applications.

Keywords:

Drug delivery LUV Lipid Lipid bilayer Liposome Phospholipids SUV Sonication Vesicle

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Preparation, Purification, and Use of Fatty Acid-containing Liposomes

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Published on: February 9, 2018

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Last Updated: Feb 27, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

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Published on: March 3, 2020

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

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Published on: December 13, 2019

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Published on: February 9, 2018

Area of Science:

Biotechnology
Materials Science
Pharmaceutical Sciences

Background:

Liposomes are versatile nanoscale vesicles with diverse applications.
Their unique physical and chemical properties have garnered significant scientific interest for decades.
Liposomes serve as crucial delivery vehicles for various substances into biological systems.

Purpose of the Study:

To provide a foundational understanding of liposome preparation and characterization.
To offer a simplified protocol for creating uniform liposomes with basic cargo.
To serve as a starting point for researchers utilizing liposomes in their studies.

Main Methods:

Outline of a simplified protocol for liposome preparation.
Methods for qualifying a uniform batch of simple liposomes.
Discussion of basic cargo incorporation.

Main Results:

A straightforward method for producing liposomes is presented.
Criteria for qualifying liposome batches are discussed.
The protocol aims for uniformity and basic cargo integration.

Conclusions:

This chapter equips readers with essential knowledge for liposome formulation.
It provides a practical starting point for researchers in diverse scientific fields.
The presented methods facilitate the development of tailored liposomes for specific research needs.