Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Real-world outcomes of BrECADD therapy in advanced-stage classical Hodgkin lymphoma: A multicentre retrospective study.

British journal of haematology·2026

Same author

Response to Comment on: "Is it time to reduce the doxorubicin dosage in Hodgkin lymphoma therapy?"

Haematologica·2026

Same author

Is it time to reduce the doxorubicin dosage in Hodgkin lymphoma therapy?

Haematologica·2026

Same author

Platelet and Fibrinogen Dynamics After CAR-T Cell Therapy in Relapsed/Refractory B-Cell Lymphoma May Predict ICANS Onset.

European journal of haematology·2026

Same author

Duration of anti-CD20 monoclonal antibody maintenance positively correlates with clinical outcomes in follicular lymphoma patients, supporting adherence to the recommended 2-year maintenance paradigm.

British journal of haematology·2025

Same author

A Comprehensive Review of Epidemiology, Risk Factors, Clinical Manifestations, Antifungal Susceptibility, and Mortality of Candida auris in Saudi Arabia.

Cureus·2025

Search research articles

Related Experiment Video

Updated: Feb 27, 2026

Magnetic Levitation Coupled with Portable Imaging and Analysis for Disease Diagnostics

Magnetic Levitation Coupled with Portable Imaging and Analysis for Disease Diagnostics

Published on: February 19, 2017

Measuring sickle cell morphology during blood flow.

Inna Kviatkovsky¹, Adel Zeidan¹, Daniella Yeheskely-Hayon¹

¹Faculty of Biomedical Engineering, Technion - IIT, Haifa, Israel.

Biomedical Optics Express

|July 1, 2017

Summary

Researchers imaged sickle cells using spectrally encoded flow cytometry to analyze their shape. This method helps understand sickle cell disease and develop new treatments for blood vessel blockages.

Keywords:

(170.1470) Blood or tissue constituent monitoring (170.1530) Cell analysis (170.1790) Confocal microscopy

More Related Videos

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Published on: November 5, 2019

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy

Related Experiment Videos

Last Updated: Feb 27, 2026

Magnetic Levitation Coupled with Portable Imaging and Analysis for Disease Diagnostics

Magnetic Levitation Coupled with Portable Imaging and Analysis for Disease Diagnostics

Published on: February 19, 2017

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Published on: November 5, 2019

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy

Area of Science:

Hematology
Biophysics
Medical Imaging

Background:

Sickle cell anemia involves red blood cells altering shape and blocking vessels during crises.
Understanding these morphological changes is key to treating sickle cell disease complications.

Purpose of the Study:

To image and analyze the 3D morphology of sickle cells.
To develop a new method for characterizing sickle cell shape.

Main Methods:

Utilized spectrally encoded flow cytometry to image blood cells from sickle cell anemia patients.
Analyzed interference patterns from cell membrane reflections.
Employed numerical simulations to model red blood cell interference patterns.

Main Results:

Developed an analytical expression for the 3D shape of sickle cells.
Compared the derived model to existing models.
Demonstrated a novel imaging approach for sickle cell analysis.

Conclusions:

The spectrally encoded flow cytometry method provides new insights into sickle cell morphology.
This technique can aid in studying the unique physiological and biomechanical properties of sickle cells.
The findings may contribute to developing more effective treatments for sickle cell disease.