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Related Experiment Video

Updated: Feb 27, 2026

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA
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Stromal interaction molecule 2 regulates C2C12 myoblast differentiation.

Tam Thi Thanh Phuong1, Tong Mook Kang1

  • 1Department of Physiology, SBRI, Sungkyunkwan University School of Medicine, Suwon, Korea.

Integrative Medicine Research
|July 1, 2017
PubMed
Summary

Stromal interaction molecule 2 (STIM2) and store-operated calcium entry (SOCE) are crucial for skeletal muscle cell differentiation. STIM2 activation controls C2C12 myoblast differentiation, highlighting its role in muscle development.

Keywords:
C2C12NFATcSOCESTIM2myoblast differentiation

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Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Physiology

Background:

  • Stromal interaction molecule 1 (STIM1)-mediated store-operated calcium entry (SOCE) is vital for skeletal muscle differentiation.
  • The role of STIM2, a STIM1 analogue, in muscle cell differentiation remains unclear.

Purpose of the Study:

  • To investigate the contribution of STIM2-mediated SOCE in C2C12 myoblast differentiation.
  • To elucidate the regulatory mechanisms involving STIM2 and its impact on muscle cell development.

Main Methods:

  • Assessed STIM2 expression and SOCE activity during C2C12 myoblast differentiation in vitro.
  • Utilized reverse transcription-polymerase chain reaction, Western blotting, and calcium imaging.
  • Examined STIM2's transcriptional regulation by nuclear factor of activated T cells (NFATc) and the effects of STIM2 knockdown on NFAT activity and differentiation.

Main Results:

  • STIM2 protein levels and SOCE activity increased during myoblast differentiation.
  • SOCE inhibition with 2-APB impaired differentiation.
  • NFATc1 overexpression elevated STIM2 expression and SOCE activity.
  • STIM2 knockdown reduced NFAT activity, SOCE, and C2C12 myoblast differentiation.

Conclusions:

  • STIM2-activated SOCE plays a significant role in controlling C2C12 myoblast differentiation.
  • STIM2 is a key regulator in the process of skeletal muscle cell development.