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Purification of Natural Antibodies Against Tau Protein by Affinity Chromatography

Michala Krestova1, Lenka Hromadkova2, Jan Ricny2

  • 1National Institute of Mental Health, Topolová 748, 250 67, Klecany, Czech Republic. Michala.Krestova@nudz.cz.

Methods in Molecular Biology (Clifton, N.J.)
|July 2, 2017
PubMed

Natural antibodies are now widely studied for their therapeutical potential. Therefore, their isolation and subsequent characterization is desired. Here, we describe an isolation method for natural anti-tau antibodies from human plasma by utilization of affinity chromatography with epoxy-activated copolymer resin. The evalution of isolation efficiency and avidity of isolated antibodies is decribed by modified indirect ELISA assay.

Keywords:
Human plasmaImmobilizationImmunopurificationImmunoreactivityIntravenous immunoglobulinNatural antibodiesTau protein

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