Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictors of a prolonged puncture-wire time in patients with ST-Elevation Myocardial Infarction (STEMI).

BMC cardiovascular disorders·2026
Same author

Detecting apple replant disease in the field - deciphering reasons for local growth depression.

PloS one·2026
Same author

Solid State Alkaline Depolymerization of Polyester Elastane Textiles in a Laboratory Kneader.

Polymers·2026
Same author

Adsorptive Decolorization of a Disodium Terephthalate Solution from Monomer Recycling of Polyester.

Polymers·2026
Same author

Editorial: Exploring volatile organic compounds in fruits and flowers: aroma, biosynthesis, and ecological impact.

Frontiers in plant science·2025
Same author

Use of a scattered light sensor for monitoring the dispersed surface in multimodal system.

Analytical and bioanalytical chemistry·2025

Related Experiment Video

Updated: Feb 27, 2026

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry
07:16

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry

Published on: February 2, 2024

1.1K

Downstream processing of hyperforin from Hypericum perforatum root cultures.

Paul Haas1, Mariam Gaid2, Ajmal Zarinwall3

  • 1Institute for Chemical and Thermal Process Engineering, Technische Universität Braunschweig, Germany; Center of Pharmaceutical Engineering, Technische Universität Braunschweig, Germany.

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V
|July 4, 2017
PubMed
Summary

This study optimized hyperforin extraction from St. John's Wort root cultures, achieving maximum efficiency in 60 minutes. Key parameters for equipment design were determined for efficient hyperforin production.

Keywords:
ExtractionHyperforinHyperforin (PubChem CID 441298)Hypericum perforatumRoot cultures

More Related Videos

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
09:48

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory

Published on: November 7, 2018

15.1K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.5K

Related Experiment Videos

Last Updated: Feb 27, 2026

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry
07:16

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry

Published on: February 2, 2024

1.1K
Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
09:48

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory

Published on: November 7, 2018

15.1K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.5K

Area of Science:

  • Phytochemistry
  • Natural Product Extraction
  • Biotechnology

Background:

  • Hyperforin is a key metabolite from Hypericum perforatum (St. John's Wort).
  • Hyperforin has been detected in hormone-induced root cultures of the plant.
  • Efficient extraction methods are needed for hyperforin production.

Purpose of the Study:

  • To develop an optimized downstream process for hyperforin-rich extract production.
  • To maximize extraction efficiency while minimizing hyperforin decomposition.
  • To determine critical parameters for industrial-scale equipment design.

Main Methods:

  • Extraction time optimization (maximum at 60 minutes).
  • Comparative analysis of rotary mixer versus stirred vessel for extraction.
  • Evaluation of nitrogen stripping for solvent evaporation to minimize decomposition.
  • Determination of specific extraction yield in a 5L stirred vessel.

Main Results:

  • Rotary mixer demonstrated higher extraction efficiency compared to a stirred vessel.
  • Stirred vessel, though capable of larger volumes, had limitations.
  • Nitrogen stripping resulted in minimal hyperforin decomposition during solvent evaporation.
  • A maximum specific extraction of 4.3 mg hyperforin/g dry weight was achieved.

Conclusions:

  • Established optimal extraction time and equipment parameters for hyperforin production.
  • Identified key factors for designing efficient extraction and evaporation equipment.
  • The study provides a basis for scalable hyperforin extract production from root cultures.